Fig. 1.

Diagrammatic representation of the NarL constructs used in this study. (a) Ribbon representation of NarL (PDB ID: 1A04), created using Pymol [51]. The RD is colored blue, the linker region in red, and the OD in yellow. The interdomain interface masks the DNA binding elements of the α9 recognition helix, while the linker blocks the α10 dimerization helix. The site of phosphorylation, Asp59, is represented by molecular spheres. The region between helices α6-α7 was too disordered to be resolved. (b) The constructs used in this study are designated by colored bars following the same color scheme as in (a). Residue range is indicated, as is the corresponding secondary structure. Arrows in the secondary structure diagram represent β-strands while cylinders represent α-helices