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. 2015 Aug 26;16(1):637. doi: 10.1186/s12864-015-1869-6

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© Li et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — bdhA is important for long-term survival of Lp in water. Weekly CFU counts of the wild-type KS79, ΔbdhA and its complement SPF236 with and without IPTG exposed to Fraquil at (a) 25 °C and (b) 37 °C. 1 mM IPTG was used to induce bdhA on the plasmid of the complemented strain. Data show the mean +/− SD of three biological replicates. DL: Detection limit. c Percentage of live, undefined or dead cells of KS79, ΔbdhA and its complement SPF236 with and without IPTG. Live/Dead staining analysed by flow cytometry was performed after the strains were exposed to Fraquil for 19 weeks at 25 and 37 °C