Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Setting up and loading 3D cell cultures. (a) Cell suspension is added dropwise on to scaffolds and incubated for 1–3 h for cell attachment, medium topped up and scaffolds incubated for cell growth. Scaffolds can be loaded from as early as 24 h after seeding. Loading may be applied by (b) vibration,55,56 (c) fluid flow,57,58,59,60,61,62 (d) compression64,65 or (e) strain.67,68
