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. 2015 Aug 27;13:51. doi: 10.1186/s12951-015-0115-3

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© Cenci et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — MIP NP SPR sensor response to Hepcidin-25. a Hepcidin-25 was injected at 7.2 pM (light blue curve), 14 pM (green curve), 72 pM (violet curve), 140 pM (orange curve) and 720 pM (red curve). The signal was recorded over timed and plotted as response units (RU). The sensorgrams of the MIP-NP-sensor response for Hepcidin-25 are shown in the inset. b Hepcidin-25 showed a log-linear response in the range 7.2–720 pM. A calibration curve was thus built for Hepcidin-25 by plotting the logarithm of the concentration vs ΔRU (Response Unit): the linear fit equation was f(x) = 6.07 ln(x) + 7.03 and the R² was 0.98