Fig. 1.

Immunohistochemical staining and quantitation of stromal ING1 using the HistoRx AQUA platform. a Representative images showing specificity of the ING1 monoclonal antibody in HEK293 cells and HEK293 cells overexpressing ING1a (left panels) and in placenta treated with or without the ING1 antibody (right panels). b Representative examples of quantitative fluorescent IHC images for ING1 expression in normal breast tissue (top row of panels) and breast cancer tissue (two bottom rows of panels). tAQUA scores represent the expression level of ING1 within the pan-cytokeratin defined epithelial/tumor compartment; sAQUA scores represent expression level of ING1 in the vimentin defined mesenchymal/stromal compartment. DAPI-stained nuclei are depicted in blue, pan-cytokeratin stained epithelial/tumor cells are depicted in green, ING1 protein expression is depicted in red and vimentin stained mesenchymal/stromal cells in white