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. 2015 Aug 26;16:49. doi: 10.1186/s12865-015-0108-x

Additional file 4: Video 2.

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The morphology of a CD4+ T cell changes from round to round-flattened during the IS formation. TCR is shown red, ICAM-1 is green, and scale bar is 2 μm. Before IS formation, TCR was uniformed on the surface of T cell. After IS formation, TCR and ICAM-1 was accumulated at the interface of T-DC.