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. Author manuscript; available in PMC: 2015 Aug 26.
Published in final edited form as: J Pathol. 2009 Feb;217(3):380–388. doi: 10.1002/path.2458

Figure 3.

Figure 3

BRCA2 protein stabilization by inhibitors of proteasomal degradation A, MCF7 cells were treated in the presence of the proteasomal inhibitor MG132 (20 μM) for 1, 2, 4 and 24 h or vehicle alone: 0 h (Left panels). Cell lysates were analyzed for the levels of total BRCA2 and Lamin by immunoblotting. B, MCF7 cells were transfected with 100 nM siRNA targeted to the E3 ligase Skp2 (Skp2), non-targeting siRNA (Neg), and mock transfected with the transfection reagent alone (Mock). The cells were allowed to adhere for 48 h before {{Ed Query please check this sentence is as meant}} transfection and collected at 48 h post-transfection. Similar results were obtained from two independent experiments.