Fig. 3.

Activation of calpain-1 in mouse retina by ON crush. a Representative western blot images of calpain-1 in protein extracts after ON crush (12 h, 1 d, 3 d, and 7 d post-injury) and control uninjured retinas of WT mice. b Densitometry analysis of western blot images showing a significant increase of calpain-1 expression at 12 h and 1 d in ON crush eyes compared to uninjured eyes. The values were normalized to β-actin and presented as the mean ± SD (n = 4). *: p < 0.05, **: p < 0.01 by unpaired, two-tailed Student’s t-test