Table 1.
Major proteins (≥0.1 %) of the quail eggshell calcified layer
| Protein | Accession (possible turkey and chicken homologs in brackets)1 | iBAQ % | iBAQ % Turkey | Chick abundance (emPAI) |
|---|---|---|---|---|
| Ovocleidin-116a g | 2898a;713b (G1N6E1;OC116) | 46.40 | 31.30 | High (65.3)2a |
| Ovalbumini | 20482;Q6V115;20852 (G1MYL1;G1MYK6;OVAL) | 15.97 | 12.52 | High (85.6)2a |
| Ovocalyxin-36a g, t | 21806 (G1N6M8;Q53HW8) | 7.80 | 6.00 | High (23.2)2a |
| Uncharacterized member of the latexin family | 11366a | 6.32 | - | - |
| Uncharacterized proteinase inhibitor (I1) | 23208 | 3.47 | - | - |
| Avidin/avidin-related proteini | 29257 (G1MSZ9;AVID) | 1.95 | 2.67 | Intermediate (2.2)2a |
| EGF-like repeat and discoidin I-like domain-containing protein 3 (EDIL3)a i | 25038;28103;20199 (G3UT43; F1NCN3) | 1.65 | 3.70 | High (83.3)2a |
| Tsukushina g, t | 7859 (G1NRF2;F1NDH7) | 1.51 | 0.25 | Intermediate (3.9)2a |
| Alpha-2-antiplasmin (SERPINF2)a | 5112;6916 (G1N0Z7;F1NAR5) | 0.60 | 0.80 | Low-intermediate2b,c,d |
| Gastric intrinsic factor | 8574 (G1MW98;R4GLQ4) | 0.42 | - | - |
| Serum albuming, t | 16345;19800;4290a (G1NCR2;ALBU;F2Z4L6) | 0.39 | 2.57 | High (113.5)2a |
| Lactadherin (MFGE8)i | 9870;11245;14016 (G1N944;E1C0K5) | 0.36 | 1.25 | High (15.9)2a |
| Glutathione peroxidase (GPX3)i, t | 19686 (G1N365;F1NPJ8) | 0.36 | 0.04 | Intermediate (2.2)2a |
| Actin(s)i | 7084 (G1NS52;ACT5;ACTG1)) | 0.36 | - | High (9.0)2a |
| Uncharacterized/similar to mucin(−5 AC) | 4106;10751;6295;6355 (G1N8Z1;E1C037) | 0.35 | 0.15 | Low (1.4)2a |
| Uncharacterized/Ovostatin-like (OVSTL) | 15509;25086;455a;9460 (G1NK51;F1NEW8) | 0.34 | 0.05 | Low (1.5)2a |
| Prostate stem cell antigen (PSCA)a t | 20430 (G1NJY7;F1NXM7) | 0.34 | 0.18 | Low (0.8)2a |
| Extracellular fatty acid-binding protein (EXFAB) | Q9I9P7 (G1MQ16;E1C0K1) | 0.34 | 3.82 | High (26.8)2a |
| Pigment epithelium-derived factor/SERPINF1i | 5860 (G1N131;E1C7H6) | 0.31 | 0.64 | Intermediate (6.0)2a |
| Clusterina i | 6174;P14018 (G1NMV6;Q9YGP0) | 0.31 | 0.31 | High (42.7)2a |
| Serpin E2/Glia-derived nexin | 6327 (G3URX3;E1BWU2) | 0.31 | 0.18 | High (11.1)2a |
| Osteopontina | Q9I832;713c (G1N6D8;G1N6D8) | 0.28 | 0.15 | High (1.89)2a |
| Apolipoprotein D (APOD) | 28649 (G1N591;Q5G8Y9) | 0.27 | 0.04 | High (18.3)2a |
| Glypican-4a | 6640 (G1MVZ0;F1NAU1) | 0.23 | 0.12 | Intermediate (6.3)2a |
| Semaphorin-3Gg, t | 621a (G1MXI6;F1NQ93) | 0.22 | 0.15 | Low (1.7)2a |
| Similar to Bactericidal/ permeability-increasing protein-like 2/BPI fold-containing family C proteina i, t | 28084;20375 (IPI_CHICK:00571823) | 0.21 | - | High (16.8)2a |
| Pentraxin (PTX3/PPTX) | 14241;14979 (G1NDX8;Q5UMH8) | 0.21 | 0.01 | Low (0.6)2a |
| Ovalbumin-related protein Xi | 30155 (G1MZH2;R9TNA6) | 0.21 | <0.01 | Intermediate (3.8)2a |
| Ovotransferrin (TRFE)i | 2596;18629;24968 (G1MVV5;E1BQC2;Q4ADJ7) | 0.19 | 2.22 | High (22.9)2a |
| Cathepsin D | 15278;3793b (G1N8P0;CATD) | 0.18 | 0.22 | Intermediate (5.3)2a |
| Cathepsin B | 8924;17826 (G1NN37;F1N9D8) | 0.18 | 0.09 | High (24.8)2a |
| Sulfhydryl oxidase 1 (QSOX1)i | 2299;5467 (G1MV84;QSOX1;F1NYK2) | 0.18 | 1.08 | Intermediate (4.7)2a |
| Apolipoprotein A-Ia g, t | 12041;P32918 (G1MVX1;APOA1) | 0.17 | 0.05 | Intermediate (2.2)2a |
| Lysozyme Cg | P00701 (LYSC;LYSC) | 0.14 | 0.64 | High (128.2)2a |
| Fibronectin (FN1) | 141;A0A060PIY8 (G1MWJ4;F1NJT3;F1NJT4) | 0.13 | 0.13 | High (18.9)2a |
| IGFBP5 | Q9DGI2 (−;F1ND88) | 0.13 | - | Low (0.6)2a |
| FAM20C kinase/DMP4a | 21781;14673 (G1MSD1;E1C4X0) | 0.12 | 0.27 | Intermediate (2.3)2a |
| Proactivator polypeptide (PSAP) | 2306;1756 (G1MZV1;E1BSP1) | 0.12 | 0.06 | High (9.0)2a |
| Polyubiquitin/ubiquitint | 11531;2058 (G1NR52;Q91021;UBB) | 0.12 | 0.12 | High (9.0)2a |
| Regenerating islet-derived protein 4 (REG4) | 8715a (G1MZE6;E1BZV4) | 0.11 | 0.38 | Low (0.7)2a |
| Cystatint | 14227 (G1N522;CYT) | 0.11 | 1.67 | High (45.4)2a |
| Ovomucoidi | P01003;15709 (IOVO;IOVO) | 0.11 | 0.05 | Intermediate (2.2)2a |
| UDP-glucuronic acid decarboxylase 1 | 11592 (G1NPP3;E1BV28) | 0.10 | 0.07 | Intermediate (2.2)2a |
| Uncharacterized/similar to mucin | 5306;6950;1527 (G1N931;E1C037) | 0.10 | 0.15 | Low (1.4)2a |
| Insulin-like growth factor-binding protein 7 (IGFBP7) | 18344 (G1N8M9;F1NVP4) | 0.10 | 0.07 | Intermediate (3.6)2a |
| Ganglioside GM2 activatora g, t | 24235 (G1N3B0;Q5ZK02) | 0.10 | - | High (12.9)2a |
| Synthenin-1 (SDCBP)t | 24420 (G1NEY6;Q5ZHM8) | 0.10 | - | Intermediate (2.8)2a |
| Similar to procollagen C-endopeptidase enhancer | 30796 (G1N443;F1NH70) | 0.10 | - | - |