Table 2. Comparison of amino acid substitutions of Usutu-BONN strain with those of all available complete Usutu virus polyprotein sequences*.
| Protein | Amino acid substitution Usutu-BONN | Unique substitutions | Total substitutions | Changed putative cleavage sites |
|---|---|---|---|---|
| C | No | No | 0/0 | No |
| anchC | S105†→G; A120→V | No | 0/2 | TKKKR/S†NNGP |
| PrM | N120→Y | N120 | 1/1 | No |
| M | N28→Y | N28 | 1/1 | No |
| E | L231→S; T238→ I/L | L231; T238 | 2/2 | No |
| NS1 | V146→ A/G | No | 0/1 | No |
| NS2A | A91→ V/T; L123→F; V142→A; V189→ A/S | V142; V189 | 2/4 | No |
| NS2B | No | No | 0/0 | No |
| NS3 | F46→ L; V338→A/T | No | 0/2 | No |
| NS4A | No | No | 0/0 | No |
| 2K | No | No | 0/0 | No |
| NS4B | F189→ L | F189 | 1/1 | No |
| NS5 | S274→T/A | No | 0/1 | No |
*USUTU-Bonn strain was isolated from a blackbird in Germany in 2014. †Substitution in cleavage sites. GenBank accession numbers of Usutu virus polyproteins used for sequence alignments are shown in Table 1.