Skip to main content
. 2015 Aug 20;8:2199–2208. doi: 10.2147/OTT.S85948

Figure 1.

Figure 1

Fluoxetine reduces MGMT expression in glioma cells.

Notes: (A) Effect of six antidepressants on MGMT protein expression in T98G glioma cells. Total cell lysate was harvested after exposure to six antidepressants (10 µM, 24 hours) and used to measure the levels of MGMT protein. (B) Real-time PCR analysis on MGMT mRNA expression following FLT treatment at indicated doses for 24 hours in T98G and U138 glioma cells. n=4, *P<0.05, as compared to no treatment control. (C) Real-time PCR analysis on MGMT mRNA expression following FLT treatment (10 µM) for indicated time interval in T98G and U138 glioma cells. n=4, *P<0.05, as compared to no treatment control. (D) MGMT protein expression following FLT treatment (10 µM, 24 hours) in T98G cells with or without GluR1 knockdown. β-actin served as a loading control.

Abbreviations: FLT, fluoxetine; MGMT, O6-methylguanine-DNA methyltransferase; PCR, polymerase chain reaction.