Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 26;10(8):e0136055. doi: 10.1371/journal.pone.0136055

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Ellefsen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 6 — Images in each panel show EGFP-tagged Orai visualized by shadowless TIRF excitation at 488 nm (top left); mCherry-tagged STIM visualized by TIRF excitation at 532 nm (top right); mCherry-tagged STIM visualized by skimming- plane (SP) excitation at 532 nm (bottom left); and a pseudocolored overlay (Orai in green, TIRF-STIM in red, SP-STIM in blue) of these three images. Images were acquired before (A) and about 3 min after (B) addition of thapsigargin to deplete ER Ca²⁺ stores and induce relocalization and clustering of Orai and STIM. Each image (81 x 61 μm) is an average of two successive exposures; brightness and contrast settings were individually adjusted for each mode, but are consistent between control and thapsigargin conditions. Traces in (C) and (D) show line-scans of EGFP-Orai (red traces) and mCherry-STIM (blue traces) fluorescence in TIRF mode obtained before from 20-frame averages captured before (C) and after (D) thapsigargin treatment.