Table 1. Primers used in polymerase chain reaction to detect IL-1β, IL-10, TNF-α, and NOS 2.
Primer name | Primer | |
---|---|---|
GAPDH | Forward | 5'-GCCCTCAATGACCACTTTGT-3' |
Reverse | 5'-TGCTGTAGCCGAACTCATTG-3' | |
IL-1β | Forward | 5'-TCCCTGTGAAAACAAGAGCA-3' |
Reverse | 5'-CGCCTTTCTCTTGGAGCTTA-3' | |
IL-10 | Forward | 5'-ACACCCAGTCTGAGGACAGC-3' |
Reverse | 5'-AAAGTCTTCACCCTGCCAAA-3' | |
TNF-α | Forward | 5'-ATCAAGAGTCCCTGCCAGAA-3' |
Reverse | 5'-CTCCCAGGTAGATGGGTTCA-3' | |
NOS2 | Forward | 5'-CCCTCTTCGTGCTGAAAAAG-3' |
Reverse | 5'-GTCATGAGCAAAGGCACAGA-3' |
IL-1β: Interleukin-1 beta, IL-10: Interleukin-10, TNF-α: tumor necrosis factor-alpha, NOS 2: nitric oxide synthase 2.