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. 2015 Aug 26;10(8):e0135638. doi: 10.1371/journal.pone.0135638

Fig 2. Alignment of LytB homologs.

Fig 2

Sequences from M. tuberculosis (M.tb), M. bovis (M.b), M. marinum (M.ma), M. smegmatis (M.sm), M. leprae (M.le), and E. coli (E.c) LytB/IspH homologues were aligned using ClustalW. M. tuberculosis LytB2, M. bovis LytB2, M. marinum IspH, M. smegmatis IspH and M. leprae LytB2 form one distinct group, with M. tuberculosis LytB1 showing a greater sequence identity with E. coli IspH, M. bovis LytB1 and M. marinum LytB2. Black circles indicate equivalents of E. coli Cys-12, Cys-96, Cys-197, required for Fe-S cluster formation [14–17,24]; the black triangle indicates the critical catalytic residue Glu-126 [26]; white inverted triangles indicate His-41, His-124, Thr-167, Ser-225, Asn-227 required for substrate binding to the active site [17,20,22,24]; black stars indicate bases changed in the LytB2 mutant alleles; white stars indicate the bases changed in the LytB1 mutant allele.