Table 1. Identification of functional alleles of LytB in M. tuberculosis.
| Plasmid | Allele | Complementation |
|---|---|---|
| pPLB2_LB1 | LytB1 under control of PlytB2 | No |
| pPLB2_LB2 | LytB2 under control of PlytB2 | Yes |
| pMS_LB | M. smegmatis LytB | Yes |
| p50 (1) | 50:50 B1:B2 fusion | No |
| p50 (2) | 50:50 B2:B1 fusion | No |
| p75 (1) | 75:25 B1:B2 fusion | No |
| p75 (2) | 75:25 B2:B1 fusion | No |
| P90 (1) | 90:10 B1:B2 fusion | No |
| p90 (2) | 90:10 B2:B1 fusion | Yes |
| pFeS.1 | LytB2 G35S | Yes |
| pFeS.2 | LytB2 D41E | Yes |
| pFeS.3 | LytB2 H62K E63Q | Yes |
| pFeS.4 | LytB2 R68T | Yes |
| pFeS.5 | LytB2 T125A | Yes |
| pFeS.6 | LytB2 V152T | Yes |
| pFeS.7 | LytB1 A121T, A125N, A128R, A132R | No |
Switching vectors containing the indicated alleles were transformed into the M. tuberculosis LytB2 del-int strain carrying an integrated versions of lytB2 associated with the gentamicin resistance gene (genotype lytB2Δ [lytB2, Gm, L5-int,]). pFeS.1-6 LytB2 base numbering, pFeS.7 LytB1. Transformants were selected on hygromycin carried by the incoming vector; gene switching was confirmed by checking for hygromycin resistance and gentamicin sensitivity.