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. 2015 Aug 26;11(8):e1005475. doi: 10.1371/journal.pgen.1005475

Fig 1. Loss of SmD1 compromises miRNA biogenesis.

Fig 1

(A) small RNA samples from various dsRNA-treated S2 cells were subject to deep sequencing. The proportions of various classes of endogenous siRNAs and miRNAs in each library are shown. Note that small RNA libraries were prepared in two batches (batch 1: control and Drosha knockdown; batch 2: two independent control samples, one Dcr-2 knockdown sample, and two independent SmD1 knockdown samples). Unless noted otherwise in this manuscript, dsRNA against the firefly luciferase gene serve as a control. (B,C,E,F) S2 cells were treated with various dsRNAs (above) and levels of the endogenous siRNA esi-2.1, various miRNAs or 2S rRNA (loading control) were measured by Northern blot. (D,G) Quantification of miRNA and esi-2.1 levels (n = 3) normalized against 2S rRNA levels and compared with controls. Unless noted otherwise, data in this manuscript are shown as mean + SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (H) Levels of various mRNA targets of the corresponding miRNAs (below) in SmD1-depleted cells or control cells were measured by RT-qPCR and normalized against the control rp49 mRNA (n ≥ 3).