Immunosensor (MC1R-Ab/n-SiNPs/PPy/SPE) response (A) in the absence (a) and in the presence of (b) 500, (c) 2500, (d) 5000, (e) 10,000 HEK-293 cells; (B) for (a) 0, (b) 100 SK-MEL-2, (c) 100 SK-MEL-2+10,000 HEK-293, (d) 1000 SK-MEL-2, (e) 1000 SK-MEL-2 +10,000 HEK-293, (f) 2500 SK-MEL-2, (g) 2500 SK-MEL-2+10,000 HEK-293, (h) 7500 SK-MEL-2, and (i) 7500 SK-MEL-2+10,000 HEK-293 cells; (C) for (a) 0, (b) 5 ×104 PBMCs+5 ×104 HEK-293 cells, and (c) 50, (d) 100, (e) 250, (f) 500, and (g) 1000 SK-MEL-2 cells each along with 5 ×104 PBMCs and 5 ×104 HEK-293 cells. (D) Linear calibration curve of anodic peak current vs. 50–1000 SK-MEL-2 cells in the presence of 5 ×104 PBMCs and 5 ×104 HEK-293 cells. Values are mean of 3 measurements, and error bars are standard deviations under optimized experimental conditions. (E) Stability of the immunosensor as a function of storage time in 1 × PBS solution at 4 °C.