Table 1.
Expression of scoC-lacZ fusions
| Strain | Fusion promoter | Relevant genotype | β-Galactosidase activity | Fold regulation | ||
|---|---|---|---|---|---|---|
| codY/codY+ | scoC/scoC+ | abrB/abrB+ | ||||
| GB1039 | scoC561p+ | wild type | 52.0 | 4.6 | 1.3 | 0.95 |
| GB1041 | codY | 241.4 | 1.8 | |||
| GB1061 | scoC | 68.8 | 6.2 | |||
| GB1063 | codY scoC | 427.2 | 0.80 | |||
| BB3897 | abrB | 49.4 | ||||
| BB3907 | codY scoC abrB | 342.4 | ||||
| BB3890 | scoC350p+ | wild type | 48.6 | 4.7 | 1.8 | |
| BB3902 | codY | 228.4 | 2.0 | |||
| BB3901 | scoC | 87.1 | 5.1 | |||
| BB3908 | codY scoC | 445.4 | ||||
| BB3898 | scoC181p+ | wild type | 120.4 | 0.94 | 1.0 | |
| BB3904 | codY | 113.5 | 1.1 | |||
| BB3903 | scoC | 120.8 | 0.99 | |||
| BB3909 | codY scoC | 119.8 | ||||
| GB1045 | scoC561p1 | wild type | 125.5 | 0.61 | 1.8 | |
| GB1046 | codY | 76.7 | 3.3 | |||
| BB4001 | scoC | 236.1 | 1.1 | |||
| BB4002 | codY scoC | 255.3 | ||||
| GB1059 | scoC561p2 | wild type | 25.1 | 1.4 | 2.6 | |
| GB1060 | codY | 34.0 | 3.2 | |||
| BB4003 | scoC | 68.6 | 1.6 | |||
| BB4004 | codY scoC | 107.5 | ||||
| BB3919 | scoC561p3 | wild type | 8.64 | 0.55 | 1.2 | |
| BB3925 | codY | 4.71 | 2.3 | |||
| BB3920 | scoC | 10.7 | 1.0 | |||
| BB3926 | codY scoC | 10.8 | ||||
Cells were grown in TSS glucose-ammonium medium with a mixture of 16 amino acids. β-Galactosidase activity was assayed and expressed in Miller units. All values are averages of at least two experiments, and the standard errors of the mean did not exceed 30%.