Table 2.
Expression of dtpT-lacZ fusions
| Strain | Fusion promoter | Relevanta genotype | β-Galactosidase activity | Fold regulation | |
|---|---|---|---|---|---|
| codY/codY+ | scoC/scoC+ | ||||
| BB3928 | dtpT279p+ | wild type | 4.30 | 0.15 | 6.9 |
| BB3929 | scoC | 29.8 | 1.0 | ||
| BB3930 | codY | 0.64 | 47.3 | ||
| BB3931 | codY scoC | 30.3 | |||
| BB4057 | scoCp1 | 1.01 | 0.89 | ||
| BB4059 | codY scoCp1 | 0.90 | |||
| BB3954 | dtpT279p1 | wild type | 7.63 | 0.14 | 4.7 |
| BB3958 | scoC | 36.2 | 0.93 | ||
| BB3957 | codY | 1.08 | 31.3 | ||
| BB3959 | codY scoC | 33.8 | |||
| BB3970 | dtpT279p2 | wild type | 0.41 | 1.2 | |
| BB3973 | scoC | 0.48 | |||
| BB3971 | dtpT279p3 | wild type | 17.6 | 0.74 | 1.1 |
| BB3975 | scoC | 20.2 | 0.96 | ||
| BB3976 | codY | 13.0 | 1.5 | ||
| BB3982 | codY scoC | 19.3 | |||
| BB3978 | dtpT279p4 | wild type | 10.5 | 0.94 | 1.1 |
| BB3983 | scoC | 11.4 | 0.86 | ||
| BB3984 | codY | 9.91 | 1.0 | ||
| BB3987 | codY scoC | 9.93 | |||
| BB4047 | dtpT84p+ | wild type | 47.8 | 0.71 | 1.2 |
| BB4049 | scoC | 56.0 | 1.1 | ||
| BB4048 | codY | 33.8 | 1.9 | ||
| BB4050 | codY scoC | 62.9 | |||
Cells were grown and β-galactosidase activity was assayed as described in Table 1.
scoCp1 is a promoter mutation; other mutations are null.