Table 3.
Expression of oppA-lacZ fusions
| Strain | Fusion promoter | Binding sites within the promoter | Relevanta genotype | β-Galactosidase activity | Fold regulation | |
|---|---|---|---|---|---|---|
| codY/codY+ | scoC/scoC+ | |||||
| BB3626 | oppA421p+ | CodY: I, II, III ScoC: I, II |
wild type | 51.0 | 0.86 | 2.6 |
| BB3628 | codY | 43.9 | 15.5 | |||
| BB3874 | scoC | 132.5 | 5.1 | |||
| BB3878 | codY scoC | 679.0 | ||||
| BB4059 | scoCp1 | 15.4 | 6.3 | |||
| BB4060 | codY scoCp1 | 96.6 | ||||
| BB3993 | oppA323p+ | CodY: II ScoC: I, II |
wild type | 251.8 | 0.30 | 1.3 |
| BB3995 | codY | 74.3 | 9.9 | |||
| BB3996 | scoC | 315.0 | 2.3 | |||
| BB3999 | codY scoC | 735.9 | ||||
| BB4029 | oppA203p+ | CodY: I, II, III ScoC: I |
wild type | 172.0 | 4.5 | 1.1 |
| BB4030 | codY | 775.4 | 1.2 | |||
| BB4031 | scoC | 181.8 | 5.0 | |||
| BB4032 | codY scoC | 915.0 | ||||
Cells were grown and β-galactosidase activity was assayed as described in Table 1.
a
scoCp1 is a promoter mutation; other mutations are null.