Table 4.
Role of TnrA in expression of the scoC561p+-lacZ fusion
| Strain | Relevant genotype | Nitrogen source | β-Galactosidase activity | Fold regulation | |
|---|---|---|---|---|---|
| Glt/NH4+ | tnrA+/tnrA | ||||
| GB1039 | wild type | NH4+ | 180.8 | 0.88 | |
| Glt | 131.4 | 0.73 | 0.59 | ||
| BB3927 | tnrA | NH4+ | 205.1 | ||
| Glt | 223.3 | 1.1 | |||
| GB1063 | codY scoC | NH4+ | 633.8 | 1.1 | |
| Glt | 849.5 | 1.3 | 1.0 | ||
| BB3967 | codY scoC tnrA | NH4+ | 587.3 | ||
| Glt | 809.3 | 1.4 | |||
Cells were grown in TSS glucose medium with 0.2% NH4Cl or 0.2% glutamate as sole nitrogen source and β-galactosidase activity was assayed as described in Table 1.