Table 6.
Role of TnrA in expression of the oppA421-lacZ fusion
| Strain | Relevant genotype | Nitrogen source | β-galactosidase activity | Fold regulation | |
|---|---|---|---|---|---|
| Glt/NH4+ | tnrA+/tnrA | ||||
| BB3626 | wild type | NH4+ | 53.5 | 0.71 | |
| Glt | 166.3 | 3.1 | 3.7 | ||
| BB3979 | tnrA | NH4+ | 75.4 | ||
| Glt | 44.8 | 0.59 | |||
| BB3874 | scoC | NH4+ | 657.7 | 0.98 | |
| Glt | 621.6 | 0.95 | 1.0 | ||
| BB3980 | scoC tnrA | NH4+ | 669.3 | ||
| Glt | 596.2 | 0.89 | |||
Cells were grown and β-galactosidase activity was assayed as described in Table 4.