Table 8.
Oligonucleotides used in this work
| Name | Sequencea | Specificity |
|---|---|---|
| Flanking forward primers | ||
| oBB67 | 5’-GCTTCTAAGTCTTATTTCC | erm (pHK23) |
| oGB26 | 5’-GGACTCTAGAGCACCTTCCTCAGGAAAGC | scoC |
| oBB576 | 5’-TCGTTGAATTCATGAAAACATCAACC | oppA735/421/323 |
| oBB697 | 5’-AATATGAATTCAGCCAATTGC | dtpT279 |
| oBB711 | 5’-GAAAAGAATTCGAAGTTTAAATATTTTAAATTG | oppA203 |
| oBB712 | 5’-TTTTCGAATTCGGAACTATTTGAAATGAG | dtpT84 |
| Flanking reverse primers | ||
| oBB102 | 5’-CACCTTTTCCCTATATAAAAGC | lacZ (pHK23) |
| oGB28 | 5’-CGGGAAAGCTTATCCTTCTCGATCGATTTCC | scoC |
| oBB577 | 5’-CGCAAAGATCTAATAATAATTTTCAGCTC | oppA735/421/203 |
| oBB698 | 5’-GCCAAAAGCTTTCTAAAAAAAGTGTA | dtpT |
| oBB701 | 5’-GCCAAAAGCTTTCTAAAAAAAGTGTA TTATAAAAATCATATTCggATATAATAC |
dtpTp1 |
| oBB702 | 5’-GCCAAAAGCTTTCTAAAAAAAGTGTA TTATAAAAATCAggTTCTTATATAA |
dtpTp2 |
| oBB703 | 5’-GCCAAAAGCTTTCTAAAAAAAGTGTA TTATAAAAATCATAggCTTATATAATAC |
dtpTp3 |
| oBB704 | 5’-GCCAAAAGCTTTCTAAAAAAAGTGTA TTATAAAAATCATATTCTTAggTAATAC |
dtpTp4 |
| oBB708 | 5’-TCTTTAGATCTTATTTTTGTAAAAAAGCTG | oppA323 |
| Internal mutagenic forward primers | ||
| oGB38 | 5’-GGTTTGTGAATTTATTAAccAATTCTTACAT | scoCp1 |
| oGB40 | 5’-GGTTTGTGAATTTATTccAAAATTCTTACAT | scoCp2 |
| oBB696 | 5’-GGTTTGTGAATTTATTAAcAAATTCTTACAT | scoCp3 |
| Internal mutagenic reverse primers | ||
| oGB39 | 5’-ATGTAAGAATTggTTAATAAATTCACAAACC | scoCp1 |
| oGB41 | 5’-ATGTAAGAATTTTggAATAAATTCACAAACC | scoCp2 |
| oBB695 | 5’-ATGTAAGAATTTgTTAATAAATTCACAAACC | scoCp3 |
The altered nucleotides conferring down mutations in the CodY- or ScoC-binding site are in lowercase. The restriction sites are underlined.