Fig 4. Inhibition of DNMT activity inhibits reformation of the basal lamina.

Trichrome staining of wounds six days post-surgery. Wounds were either untreated (A, B; mock; n = 4); received an implanted bead containing 2’deoxycytidine without a deviated nerve (C, D; 2’dC; n = 8); received a surgically deviated nerve to induce formation of an ectopic blastema (E, F; NDev; n = 4); or received an implanted bead containing decitabine without a deviated nerve (G, H; Dec; n = 8). Images in (B, D, F and H are higher magnifications of the boxed areas in (A, D, E, and G correspondingly). In order to increase the visibility of the basal lamina (stained blute) in the original images, the high magnification images (panels B,D,F,H) have been color adjusted by placing the mid-tone colors on the blue end of the spectrum and highlights on the yellow end of the spectrum. All four panels were adjusted as one image, and thus have been treated equally. The lower magnification images were not adjusted. Dotted lines indicate the transition between the uninjured skin (right) and the wound (left). Arrows indicate the region beneath the epidermis/WE where the basal lamina structure can be detected. Scale bars = 200 microns.