Fig 1. Selection of candidate cDNA clones encoding human N-myristoylated proteins from a cDNA resource.

A. Strategy for selection of candidate cDNA clones encoding human N-myristoylated proteins from a cDNA resource. B. Schematic representation of the generation of NNNNN-tActin-FLAG with N-terminal 10 amino acid sequences of the ORFs of KOP cDNA clones at its N-terminus. C. Detection of protein N-myristoylation of NNNNN-tActin-FLAGs by metabolic labeling in an insect cell-free protein synthesis system. NNNNN-tActin-FLAGs were synthesized in the presence of [³H]leucine or [³H]myristic acid, and the labeled translation products were analyzed by SDS-PAGE and fluorography. The myristoylated samples are indicated by red circles. The results of the predictions of protein N-myristoylation using two prediction programs, The MYR Predictor and Myristoylator, are shown in the lower panels. R, T, and blank represent ‘Reliable’, ‘Twilight zone,’ and ‘No’ prediction from The MYR Predictor, respectively. H, M, L, and blank represent ‘High confidence’, ‘Medium confidence’, ‘Low confidence,’ and ‘No’ predictions from Myristoylator, respectively.