Fig 2. Detection of protein N-myristoylation of the gene products of 19 full-length cDNAs by metabolic labeling in an insect cell-free protein synthesis system and in transfected HEK293T cells.

A. The gene products of 19 full-length cDNAs, in which efficient incorporation of [³H]myristic acid was observed with the tActin fusion proteins, were synthesized using an insect cell-free protein synthesis system in the presence of [³H]leucine or [³H]myristic acid. The labeled translation products were analyzed by SDS-PAGE and fluorography. Faint bands are indicated by arrowheads. B. The 19 full-length cDNAs analyzed in Fig 2A were transfected into HEK293T cells, and metabolic labeling with [³H]myristic acid was performed. The labeled translation products were separated by SDS-PAGE and then analyzed by western blotting using an anti-FLAG antibody or fluorography. The samples that showed no protein expression are indicated by blue circles in the upper panels. The samples in which protein N-myristoylation was observed are indicated by red boxes in the lower panels. Faint bands are indicated by arrowheads.