Fig 5. Endogenous SAMM50 expressed in COS-1 cells is N-myristoylated.

Metabolic labeling of endogenous SAMM50 expressed in COS-1 cells with [³H]myristic acid was performed. Tag-free-SAMM50 exogenously expressed in COS-1 cells was used as a control. A. Expression of exogenously expressed Tag-free-SAMM50 and endogenous SAMM50 in COS-1 cells was determined by western blotting analysis using an anti-SAMM50 antibody. B. Protein N-myristoylation of exogenously expressed Tag-free-SAMM50 and endogenous SAMM50 in COS-1 cells was determined by [³H]myristic acid labeling. MW; molecular weight marker, IP; immunoprecipitated with anti-SAMM50 antibody. Upper and lower panels show the result of C.B.B.-staining and fluorography, respectively. Arrowheads in the upper panel indicate the position of heavy chain of IgG used for immunoprecipitation.