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. 2015 Jun 11;29(9):3911–3919. doi: 10.1096/fj.15-271783

Figure 1.

Figure 1.

Kinetics of C22→C24 PUFAs. A) 22:5n-6 incubations. FADS2, FADS1, and control stable MCF-7 cells were incubated with 22:5n-6 at 50 μM and sampled at 3, 6, 12, 18, and 24 h. In all cells, 24:5n-6 starts to appear at 3 h and increases to 24 h, rising in parallel with 22:5n-6 (right y axis) showing that 24:5n-6 is a product of 22:5n-6. B) 22:6n-3 incubations. FADS2, FADS1, and control stable MCF-7 cells were incubated with 22:6n-3 at 50 μM. Analogous to (A), the time-dependent increase in 24:6n-3 is seen in all 3 cell types, showing that 24:6n-3 is a product of 22:6n-3.