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. 2015 May 18;29(9):3702–3712. doi: 10.1096/fj.15-270645

Figure 6.

Figure 6.

Inhibition of γ-secretase has no effect on the expression of EGFR. A) WT PCNC were grown in 6-well plates for 9 d and then treated with γ-secretase inhibitor L-685,458 (500 nM) overnight (+). WT control (−) cultures were treated with the same solution without the inhibitor. After incubation, lysates were collected and assayed on WBs for the indicated proteins. (Bottom) Densitometric analysis of the relative amounts of EGFR and APP-CTFs in PCNC normalized to actin and expressed as the percentage of the amounts in nontreated controls. Data were from 4 independent experiments. ***P < 0.001 (paired Student’s t test). B) Cultures of iMEF cells were treated overnight with γ-secretase inhibitor L-685,458 (+) as above or with medium without inhibitor as controls (−). After incubation, lysates were collected and assayed on WBs for indicated proteins. (Bottom) Densitometric analysis of the relative amounts of EGFR and APP-CTFs in iMEF cells as above. Data are from 4 separate experiments as above.