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Ang-4 promotes vascular permeability in vivo. Blood vessel permeability was measured in T241 tumor-associated vessels using multiphoton microscopy and dorsal skinfold chambers. Ang-4- and VEGF-C-expressing tumors showed marked elevations in vascular permeability. Four to 7 live animals were imaged using multiphoton microscopy. Statistical analysis was performed using 1-way ANOVA and Tukey’s HSD post hoc test. *P < 0.05.
