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. 2015 Jun 9;29(9):3626–3637. doi: 10.1096/fj.15-272450

Figure 4.

Figure 4.

P2X7 receptor signaling on macrophages is anti-inflammatory. A–E) Blood and lavage fluid were obtained from WT→WT and P2X7-KO→WT bone marrow chimeric mice 16 h after CLP, and cytokines were measured by ELISA. *P < 0.05; **P < 0.01 vs. WT→WT bone-marrow chimeras (P2X7 WT→P2X7 WT and P2X7 KO→P2X7 WT; n = 8 and 7, respectively). F–J) CLP-induced sepsis increased cytokine levels in WT recipients of adoptively transferred P2X7-KO macrophages more than in WT recipients of WT macrophages (Mϕ). Cytokines were measured 16 h after CLP. *P < 0.05; **P < 0.01 vs. WT Mϕ→WT transfer (n = 10-10 recipient mice in each group). K–P) P2X7fl/−-LysM-Cre+/− and P2X7fl/−-LysM-Cre−/− mice were interbred to produce P2X7fl/fl-LysM-Cre+/− (P2X7fl/fl -LysM-Cre) mice and control P2X7fl/fl-LysM-Cre−/− (P2X7fl/fl) mice. Blood and peritoneal lavage fluid were collected at 16 h after CLP. *P < 0.05; **P < 0.01 vs. P2X7fl/fl (P2X7fl/fl and P2X7fl/fl-LysM-Cre mice; n = 8 and 10, respectively). Data are expressed as means ± sem.