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. 2015 Aug 26;10(8):e0136313. doi: 10.1371/journal.pone.0136313

Fig 5. Depletion of Atg5 inhibits IDN5706-induced autophagy, but not EDEM1 degradation.

Fig 5

H4 cells stably expressing either luciferase shRNA (control; shLuc) or Atg5 shRNA (shAtg5) were treated with 250 μM IDN5706 for different periods of time. Cellular extracts were subjected to SDS-PAGE followed by Western blot with antibody to LC3, EDEM1 or Atg5-Atg12 complex. LC3-I, non-lipidated LC3; LC3-II, lipidated LC3; mEDEM1, mature EDEM1. The asterisk indicates a band detected only in H4 cells. Western blotting with antibody to β-actin was used as loading control. The position of molecular mass markers is indicated on the left.