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. 2015 Aug 26;10(8):e0135353. doi: 10.1371/journal.pone.0135353

Fig 2. Lack of essential PUFAs leads to a decrease in the visual positive phototaxis behavior.

Fig 2

(A) Flies raised on the low-PUFA diet show reduced response to light in the positive phototaxis test when compared to flies which were raised on the high-PUFA diet (t-test; ****p < 0.0001). This defect could not be rescued by adding non-essential fatty acids (C18:0 or C18:1) (1-Way-ANOVA; ****p < 0.0001). Adding either oils (WO or LO) that contain both C18:2 and C18:3 in different ratio or single essential PUFAs (C18:2 or C18:3) rescued positive phototactic behavior (1-Way-ANOVA; ****p < 0.0001 or p*** < 0.001, respectively), The observed reduction in phototactic behavior is also rescued when flies were raised on the low-PUFA diet, and then were fed with low-PUFA diet + C18:3 for 48h only during adulthood (KW-test; ***p < 0.001) (N = 25). The significance levels indicate the results of the applied post-hoc tests. Detailed information about statistical values can be found in S3A Table (B) The basal locomotory test shows that the visual phenotype observed in A is not due to general locomotory impairments. Data represent mean ± SEM (N = 18, 1-Way-ANOVA or KW-test; ns: p > 0.05). Detailed information about statistical values can be found in S3B Table.