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. 2015 Aug 26;10(8):e0134268. doi: 10.1371/journal.pone.0134268

Fig 3. Effects of RNAi on the p21 expression in the ASCs.

Fig 3

GFP expression was monitored under a fluorescent microscope 48h after lentiviral infection. A and B, the p21-shRNA-infected ASCs. C and D, the empty-vector-infected ASCs. Fluorescence was clearly observable in both the p21-shRNA-infected ASCs (3A) and the empty-vector-infected ASCs (3C). Cells from both groups were healthy. E: RT-qPCR of the p21 expression. Note that both of the target sequences (S1 and S2) significantly down-regulated p21 mRNA expression in the ASCs (P<0.05). No significant difference was found between the empty-vector-iRNA ASCs and the untreated ASCs (P>0.05).