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. 2015 Aug 26;10(8):e0134268. doi: 10.1371/journal.pone.0134268

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© 2015 Guo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — A and B: β-Galactosidase staining. A, S1-iRNA ASCs. Note these cells were barely stained, changes in cell volume were not detectable. B, Empty-vector-iRNA ASCs. Note most of these cells were stained in deep blue (senescent cells), with an increase in cell volume. C, Comparisons between the S1-iRNA ASCs, the empty-vector-iRNA ASCs and the untreated ASCs in the percentage of the senescence positive ratio of the cells. Note that senescent rate of the cells infected by S1-siRNA was significantly lower than those of the other two groups (P<0.05).