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. 2015 Aug 26;10(8):e0136328. doi: 10.1371/journal.pone.0136328

Fig 2. Determination of the binding regions of mAbs by Western blotting.

Fig 2

Four serotypes of DENV antigen (D1 to D4) from infected C6/36 cell lysates were analyzed and detected with mAbs by Western blotting. (A) The E proteins (about 53 kDa) of DENV4 were recognized by DD1-4, DD4-3, DD5-1, DD9-4, DD13-4, DD17-4, DD27-8, DD30-4, and DD31-3. (B) The E proteins of DENV3 and DENV4 were recognized by DD14-1 and DD15-2. The E proteins of DENV1-4 were recognized by DD11-4 and DD18-5. (C) The dimeric form of the NS1 proteins (about 75 kDa) was recognized by DD3-7, DD7-8, and DD33-2. DB16-1 was used as a positive control for dimeric NS1 proteins. (D) DD1-4, DD11-4, DD13-4, DD14-1, DD18-5, DD30-4, and DD31-3 recognized the EDI-II protein (about 36 kDa) of DENV4. The 4G2 mAb (which targets the EDI-II protein of DENV4) was used as a positive control. NMIgG was used as a negative control. (E) DD17-4 and DD27-8 recognized the EDIII protein (about 17 kDa) of DENV4. NMIgG was used as a negative control.