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. 2015 Aug 26;10(8):e0136328. doi: 10.1371/journal.pone.0136328

Fig 4. In vitro ADE of DENV mediated by cross-reactive mAbs.

Fig 4

(A) K562 cells were incubated with DENV1 (MOI = 1), DENV2 (MOI = 1), DENV3 (MOI = 5), or DENV4 (MOI = 1) in the presence of the serially-diluted mAbs; the percentage of infected K562 cells was examined at 72 hours post-infection by staining with 4G2, followed by flow cytometry. (B) K562 cells were incubated with DENV1-4 and the indicated mAbs at 100 μg/ml, and the percentage of infected K562 cells was determined at 72 hours post-infection by staining with 4G2, followed by flow cytometry. NMIgG was used as a control. Data shown are from one representative experiment of two independent experiments. Unpaired Student’s t tests were used to calculate P values (**P<0.01, ***P<0.001).