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. 2015 Aug 26;10(8):e0136328. doi: 10.1371/journal.pone.0136328

Fig 5. Evaluation of in vitro and in vivo ADE with cross-reactive mAbs.

Fig 5

(A) DENV2 S221 (MOI = 5) was incubated with the serially-diluted mAbs for 1 hour at 4°C and then used to infect K562 cells. After 3 days, the cells were stained with 4G2, and analyzed by flow cytometry. (B) K562 cells were incubated with DENV2 S221 (MOI = 5) and the indicated mAbs at 100 μg/ml, and the percentage of infected K562 cells was determined at 72 hours post-infection by staining with 4G2, followed by flow cytometry. NMIgG was used as a control. Unpaired Student’s t tests were used to calculate P values (**P<0.01). (C) AG129 mice were i.v. infected on day 0 with 1 × 105 pfu DENV2 S221 and i.p. treated on days -1 and 1 with 5 μg of DD11-4 (n = 7), DD18-5 (n = 7), or NMIgG (n = 8). The survival rates were recorded for 30 days. Kaplan-Meier survival curves and P values are shown (***P<0.001, compared to NMIgG). Data shown are from one representative experiment of two independent experiments.