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. 2015 Aug 26;10(8):e0136328. doi: 10.1371/journal.pone.0136328

Fig 6. Identification of the epitope residues on E protein.

Fig 6

BHK-21 cells were transfected with vectors expressing wild-type or mutant DENV4 E proteins. After 2 days, the cells were reacted with DD11-4 (A) or DD18-5 (B), and then analyzed by flow cytometry. The binding index of a mAb to a mutant E protein was measured. The binding indices of DD11-4 and DD18-5 were reduced by mutations at W212 and E26, respectively. Data shown are from one representative experiment of two independent experiments. (C and D) Various wild-type or mutant DENV1-3 E proteins were expressed in BHK-21 cells. After fixation and permeabilization, the cells were incubated with mAbs, and analyzed by flow cytometry. The binding index of a mAb to a mutant E protein was measured. Mutations of W212 (W210 in DENV3) and E26 led to a significant loss of binding activity of DD11-4 (C) and DD18-5 (D), respectively. (E) The epitope of DD11-4 is located at residue W212 in EDII. (F) The epitope of DD18-5 is located at residue E26 in EDI. The model is based on the DENV2 E protein model (PDB: 1OAN). EDI is shown in red, EDII is shown in yellow, and EDIII is shown in blue.