Fig. 2.

Schematic representation of the JL-CK virus variants plaque-purified from the JL-CK vaccine. The variants are numbered JL-CK1-5 in order of increasing number of mutations. Locations where the sequence differs from the published RIT4385 genome are indicated. Those positions on the genome which are linked are marked *(G/A^nt92, C244R and E569K), ^#(N56T, P93S, D311G) and ⁺(V529A, L542L, L557P, F295S). The K36R mutation was identified in plaques, it was not identified by Sanger sequencing of the unpassaged JL-CK vaccine. The Ter550Q mutation results in an extended nucleoprotein open reading frame, incorporating the L557P mutation. The ratio of the different subpopulations is expressed as a percentage of the 81 plaques isolated from Vero cells and Sanger sequenced.