Fig. 3.

(A) Progeny virus titration on Vero cells after a 7 day timecourse assay on Vero cells. Viruses were inoculated at a MOI 0.0001. Progeny viruses were titrated in triplicate and plotted as pfu/ml of cell lysate. (B) Measurement of plaque size of JL-CK, RIT4385 and JL-CK variants using ImageJ. The “Parent” panel is the isolated parent-like variant which has the identical sequence to RIT4385. The data plotted is the number of plaques with corresponding plaque size, measured in ImageJ units.