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Neurovirulence scoring from neonatal rat brains 25 days after inoculation with JL-CK, RIT4385 or JL-CK variants. The data shown is from six brains from each group, with measurements taken from four sections of each brain. An increase in neurovirulence compared to RIT4385 was observed for JL-CK1 (*p = 0.0406) and JL-CK5 (**p = 0.0100). JL-CK displayed an increase in neurovirulence potential compared to the parent-like variant isolated from JL-CK (***p = 0.0440).
