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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biomaterials. 2015 Jul 17;67:183–193. doi: 10.1016/j.biomaterials.2015.07.033

Figure 3. Effect of TDP-A on cell cycle progression, production of ROS and mitochondrial integrity in MDA-MB-231 cells.

Figure 3

(A) Cells were treated with different concentrations of TDP-A for 24 h. The cell cycle analysis was conducted by FACScan flow cytometry. (B) Western blot analysis of p21 and cyclin D1 expression after TDP-A treatment for 24 h or 48 h. (C) TDP-A induced the production of ROS in MDA-MB-231 cells. Intracellular ROS was analyzed by flow cytometry after DCFDA probe staining. (D) TDP-A increased the mitochondrial membrane potential (MMP) as assayed by DiOC6 dye staining.