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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Chromosoma. 2015 Apr 18;124(3):397–415. doi: 10.1007/s00412-015-0511-3

Table 1. Primary Antibodies Used in this Study*.

Antibody Host Producer Cat.
Number/Reference		 Dilution
IF WB
PRDM9 G. Pig Custom-made N/A 1:100 1:5000
FLAG Rabbit Sigma F7425 1:200 n/a
KIT Rabbit Millipore CBL1360 1:100 n/a
STRA8 Rabbit Abcam Ab49405 1:200 n/a
SMC1A Mouse Watanabe Lab Ishiguro et al. 2011 1:500 n/a
RAD21L Rabbit Watanabe Lab Ishiguro et al. 2011 1:500 n/a
REC8 Rabbit Watanabe Lab Ishiguro et al. 2011 1:500 n/a
BRCA1 Rabbit Namekawa Lab Ichijima et al. 2011 1:100 n/a
ATR Rabbit GeneTec GTX74211 1:200 n/a
MDC1 Sheep SeroTex AHP799 1:1000 n/a
SYCP1 Rabbit Novus NB 300-229 1:100 n/a
SUMO1 Rabbit Cell Signaling 4390 1:100 n/a
SYCP3 Rat Handel Lab Eaker et al. 2001 1:1000 n/a
HIST1H1T G. Pig Handel Lab Cobb et al. 1999 1:500 n/a
RAD51 Rabbit CalBiochem PC130 1: 200 n/a
P-H2AFX Rabbit Millipore 07-146 1:200 n/a
HORMAD1 Rabbit GeneTex GTX119236 1:100 n/a
TEX12 Rabbit PTG 17068-1-AP 1:200 n/a
H3K4me3 Rabbit Millipore 07-743 n/a n/a
MLH1 Mouse BD Pharmingen 51-1327GR 1:50 n/a
POLII Mouse Abcam Ab817 1:200 n/a