Fig. 1.

MHV E localizes in the ERGIC and Golgi. Mouse 17Cl1 cells were infected with MHV A59 at a MOI of 1 and analyzed at 6 h p.i. (A) Cells were processed for dual-label immunofluorescence detection of E and protein disulfide isomerase (PDI), ERGIC-53, or mannosidase II (Mann-II), as ER, ERGIC, and Golgi markers, respectively. The bottom panels were probed for M and E proteins using rabbit polyclonal anti-E 9410 and mouse monoclonal J1.3 and J2.7 antibodies, respectively. Alexa-Fluor tagged secondary antibodies were used to counter stain the primary antibodies. Merged images are shown in the far right column, with enlarged insets of selected cells. Epifluorescence images were taken using a 60× objective. (B) Cells were processed for dual-label immunofluorescence detection using antibodies specific for E (9410) and cis Golgi GM 130, medial Golgi Mann-II or trans Golgi p230. Confocal images were taken with a 100× objective. Merged images are shown in the far right column, with enlarged insets of selected cells. Scale bar, 5 μm (A, B).