Fig. 5.

MHV recombinant with TC-tagged E. (A) 17Cl1 mouse cells were infected with WT or MHV E-TC viruses at a MOI of 1. Cells were labeled with Lumio Green™ at 8 h p.i., fixed and stained for E with antibody 9410. Yellow in the merged panels indicates colocalization of TC-Lumio™ Green and E. (B) Growth kinetics and plaque characteristics of WT and MHV E-TC viruses were analyzed in 17Cl1 cells infected at a MOI of 0.01. Titers were determined by plaque assay on L2 cells at the indicated times. Growth kinetic titers represent the average of two independent experiments from plaque assays performed in duplicate. Scale bar, 5 μm.