. 2015 May 6;22(17):13546–13555. doi: 10.1007/s11356-015-4597-1

Table 1.

20 % and 50 % lethal concentration (LC₂₀ and LC₅₀ with 95 % upper and lower confidence limits) of H. discus hannai Ino in different NiCl₂ concentrations at 22 and 26 °C for 96 h calculated by probit analysis

Water temperatures (°C)	Probit analysis	Estimated values (mg/L)	95 % confidence limit
Water temperatures (°C)	Probit analysis	Estimated values (mg/L)	Upper limit	Lower limit
22	LC₂₀	9.929	−21.196	22.637
22	LC₅₀	28.591	14.747	49.526
26	LC₂₀	4.116	−5.428	8.169
26	LC₅₀	11.761	7.588	16.764

Control and NiCl₂ concentration lower than 5 mg/L did not have any mortality until the end of the exposure periods