. 2015 May 6;22(17):13546–13555. doi: 10.1007/s11356-015-4597-1

Table 2.

Biochemical analysis of hemolymph in H. discus hannai Ino in different NiCl₂ concentrations at 22 and 26 °C for 96 h

	Water temperatures (°C)	Exposure concentration (μg/L)
	Water temperatures (°C)	Control	100	400
Ca (mg/dL)	22	4.270 ± 0.389^a	5.506 ± 0.065^ab	6.180 ± 0.324^ab
Ca (mg/dL)	26	6.854 ± 0.065^b	9.438 ± 1.557^c	9.438 ± 0.778^c
Mg (mg/dL)	22	13.102 ± 0.386^c	12.834 ± 0.618^c	13.235 ± 0.463^c
Mg (mg/dL)	26	11.832 ± 0.039^bc	10.829 ± 0.618^ab	10.361 ± 0.039^a
Total protein (g/dL)	22	2.416 ± 0.005^a	2.426 ± 0.001^a	2.417 ± 0.005^a
Total protein (g/dL)	26	2.508 ± 0.037^b	2.453 ± 0.005^ab	2.407 ± 0.001^a
Glucose (mg/dL)	22	26.238 ± 0.286^a	26.733 ± 0.001^ab	27.228 ± 0.286^ab
Glucose (mg/dL)	26	27.723 ± 0.286^b	27.475 ± 0.661^b	27.227 ± 0.285^ab
ALP-S (K-A)	22	6.316 ± 0.058^a	6.391 ± 0.043^ab	6.541 ± 0.043^b
ALP-S (K-A)	26	6.391 ± 0.043^ab	6.541 ± 0.043^b	6.466 ± 0.058^ab

Each value represents a mean value ± SD of three replicates (n = 10). Values with different superscripts are significantly different (P < 0.05) as determined by Duncan‘s multiple range test