FIG 1.

Optimization of Adjuplex and antigen dose. (a) Mice (C57BL/6, 3 per group) were immunized on days 0 and 14 with HA in Adjuplex, and blood was taken on days −1, 14, and 28. (b) Total antigen-specific IgG production quantified by AUC analysis. (c) Hemagglutination inhibition (HI) titers were measured and expressed as log₂ values. (d) Weight measured over 48 h and plotted as the percent change from starting weight (100%). (e) Weight loss quantified by AUC. (f) The immunogenicity-to-toxicity ratio was determined by multiplying weight loss by the IgG titer. Data represent the mean ± standard error of the mean (SEM). *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s., not significant.