FIG 2.

Comparison of relative activity of Adjuplex and other commonly used adjuvants. (a) Mice (C57BL/6, 5 per group) were immunized on days 0 and 18 with HA alone, Adjuplex alone, or HA in Adjuplex, alum, or MPL; blood was taken on days −1, 10, and 36, and HA-specific IgG titers were determined. (b) HA-specific IgG titers quantified by AUC analysis. (c) Hemagglutination inhibition (HI) titers measured and expressed as log₂ values. (d) Weight measured over 48 h and plotted as the percent change from starting weight (100%). (e) Weight loss quantified by AUC analysis. (f) Box (interquartile range) and whisker (complete range) plots of toxicity relative to adjuvanticity for each condition, quantified by determining the ratio of weight loss to IgG titer. (b to e) Data represent the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; n.s., not significant.